Study on mechanism of Qinbaohong Oral Liquid in anti-inflammation, stopping cough, eliminating phlegm and relieving asthma based on network pharmacology / 中国中药杂志

This study aimed to investigate the anti-inflammatory, antitussive, expectorant, and anti-asthmatic effects of Qinbaohong Oral Liquid in mouse experiments and explore its action mechanism based on network pharmacology. The mouse auricle swelling was induced by xylene for detecting the anti-inflammatory effect of Qinbaohong Oral Liquid, whose antitussive effect was then examined in mice with cough after exposure to ammonium hydroxide. The expectorant effect was determined based on the excretion of phenol red into the mouse trachea. The mouse model of asthma induced by histamine phosphate and acetylcholine chloride was used to observe the anti-asthmatic effect. The chemical components of Qinbaohong Oral Liquid were retrieved from TCMSP and literature, followed by target prediction based on BATMAN-TCM. The targets of inflammation, cough, expectoration, and asthma collected from GeneCards were intersected with drug targets for GO and KEGG enrichment analysis using Metascape. The results were imported into STRING for exploring protein-protein interactions and screening the key targets. As demonstrated by our findings, Qinbaohong Oral Liquid at 4.5 and 9.0 mL·kg~(-1) obviously decreased the weight(P<0.05) and thickness(P<0.01) of the right swelling ear and also the weight diffe-rence(swelling degree) between the two ears(P<0.05), prolonged the incubation period of cough(P<0.05), reduced the frequency of cough within 3 min(P<0.05), and increased the excretion of phenol red into the mouse trachea(P<0.01). Qinbaohong Oral Li-quid at 2.3, 4.5, and 9.0 mL·kg~(-1) dramatically prolonged the incubation period of asthma(P<0.05). A total of 324 chemical components and 1 245 targets were harvested for the Qinbaohong Oral Liquid, together with 10 272 inflammation targets, 4 400 cough targets, 192 expectoration targets, and 7 533 asthma targets. Their intersection revealed that the anti-inflammatory, antitussive, expectorant and anti-asthmatic effects of Qinbaohong Oral Liquid were correlated with such GO biological processes as the regulation of ion transport and blood circulation and such KEGG pathways as cancer-related signaling pathways and neuroactive ligand-receptor interaction. Qinbaohong Oral Liquid has been confirmed by both experiments and network pharmacology analysis to be efficient in anti-inflammation, stopping cough, eliminating phlegm, and relieving asthma.

Effect of San'ao Decoction on ovalbum induced asthmatic mice and expression of TRPV2 in lung / 中国中药杂志

To observe the efficacy of San'ao Decoction(SAD) in diffusing the lung and relieving asthma, and its intervention effect on the expression of transient receptor potential V2(TRPV2) during alleviating asthma, this study replicated an ovalbumin(OVA)-induced asthmatic mice model, and investigated the intervention effect of SAD on the airway inflammation and airway hyperresponsiveness. The regulatory mechanisms of SAD on the mRNA and protein expressions of TRPV2 in lung tissues and the levels of interleukin-4(IL-4),-10(IL-10), nerve growth factor(NGF), prostaglandin D_2(PGD_2) in bronchoalveolar lavage fluid(BALF) were discussed. Compared with the control group, the model group showed typical asthmatic phenotype, the level of eosinophils(EOS) in peripheral blood and BALF as well as the airway hyperresponsiveness were increased(P<0.01), and pathological damage in lung tissue was serious. The mRNA and protein expressions of TRPV2 in lung tissue were increased significantly, while the levels of IL-4, IL-10, NGF and PGD_2 in BALF were elevated(P<0.05,P<0.01). SAD could relieve bronchial asthma manifested as repaired lung patholo-gical changes(P<0.05), reduce the level of EOS in blood and BALF(P<0.05, P<0.01), and improve pulmonary resistance and lung compliance(P<0.05, P<0.01). SAD could also regulate the inflammatory cytokine levels of IL-4, IL-10, NGF, PGD_2 in BALF, and reduce the gene and protein expression of TRPV2 in the lung tissue(P<0.05, P<0.01). It is verified that SAD could reduce the lung inflammation, and improve lung function in asthmatic mice. The regulatory mechanism of SAD on asthma induced by OVA might be related to the regulation of TRPV2 expression and the induced decrease of Th2-related cytokines and neuropeptides, which provides the evidences for the treatment of asthma with SAD.

Study on Cough-preventing and Asthma-relieving Effect of Shaonianhong Fanxing Anti-asthmatic Syrup / 国际中医中药杂志

Objective To observe the cough-preventing and asthma-relieving effect of Shaonianhong Fanxing Anti-asthmatic Syrup(SFAS).Methods The cough-preventing effect was observed in the experiment of cough induced by ammonia in mice;the phlegm-removing effect was observed in the experiment of eliminating phlegm with phenol red in mice;and the and-asthmatic effect was observed in the experiments of histamine inducing asthma and isolated tracheal smooth muscles of cavies.Results SFAS Can prolong the coughing time induced by ammomia,enhance the excretion of sputum,extend the delitescence period of ohosphoric acid hismmine induced asthma and restrain the contraction of isolated tracheal smooth muscles.Conclusion SFAS has phlegm-removing,cough-preventing and anti-asthmatic effects.

Effect of "Dispersing Lung-qi and Relieving Asthma,Warming Yang to Induce Diuresis" Method on ADH of CPHD Patients during Acute Period with Peripheral Edema / 中国中医药信息杂志

Objective To evaluate the effectiveness of "dispersing lung-qi and relieving asthma, warming Yang to induce diuresis" method on CPHD patients during acute period with peripheral edema and observe the effect on ADH. Methods The CPHD patients during acute period with peripheral edema were randomly divided into treatment group and control group. The treatment group received Chinese medicine adding to regular western medicine, the principle is dispersing lung-qi and relieving asthma, resolving phlegm to stop coughing, warming Yang to induce diuresis. The control group received placebo adding to regular western medicine. Both of the groups were treated for 14 days. The effect observed indexes were as follows:general curative effect, integral of main symptoms of TCM, integral of western medical signs, serum electrolyte, etc. Mechanism index was about ADH. Results The treatment group had better effects than the control group on the general curative effect, reducing integral of symptoms and signs, rectifying electrolyte and acid-alkaline-balance disturbances, etc. The safety analysis of two groups had no significant abnormality. Meanwhile, the treatment group had more superiority than the control group on reducing serum ADH. Conclusion The comprehensive treatment of traditional Chinese and western medicine probably has more advantage in increasing the general effect, improving symptoms and signs, rectifying electrolyte and acid-alkaline-balance disturbances, etc. This therapeutic method can restrain secretion of ADH. It indicates that restraining secretion of ADH maybe part of the mechanism of using "dispersing lung-qi and relieving asthma, warming Yang to induce diuresis" method to treat CPHD patient during acute period with peripheral edema.

Treatment of Asthma at Asthma-relieving Earpoint and its Influence on FEV1 and PEF / 针灸推拿医学（英文版）

Purpose: To investigate the treatment of asthma at asthma-relieving earpoint and its influence on FEV1 and PEF. Method: One hundred and seventy-eight patients exactly diagnosed with bronchial asthma were selected and averagely divided, according to a simple random number table, into an asthma-relieving earpoint treatment group (observation group)and a body acupuncture treatment group (control group). Results: The effective rate was 90.0% in the observation group and 91.0% in the control group. A comparison of the curative effects between the two groups showed P＞ 0.05. In the observation group there were significant differences in FEV1 and PEF between pretreatment and posttreatment (P＜ 0.05 and P＜ 0. 01, respectively). Conclusion: Asthma-relieving earpoint is markedly effective in treating asthma and has a certain influence on lung function.

Effect of Tianyizhike Syrup on dispelling phlegm and relieving asthma / 中草药

Object To observe the effect of Tianyizhike Syrup on dispelling phlegm and relieving asthma. Methods The phenol red secreting tests in mice, capillary method in rats, asthma induced by spraying and trachea screwy strip method on guinea pig were adopted for observing the related pharmacological effect in different doses of Tianyizhike Syrup. Results Tianyizhike Syrup could obviously increase the amount of the mice secreting phenol red and the rat dispelling phlegm, prolong the latent period of the guinea pig asthma and significantly enlarge the bronchia smooth muscle. Conclusion Tianyizhike Syrup has a significant effect of dispelling phlegm and relieving asthma.